CRISPR genome editing in mice

​CRISPR-mediated mouse genome editing is typically accomplished by microinjection of Cas9 DNA/RNA and sgRNA into zygotes to achieve editing in one step. We developed a simple and economic electroporation-based strategy, designated as  CRISPR RNP Electroporation of Zygotes (CRISPR-EZ), to deliver Cas9/sgRNA ribonucleoproteins (RNPs) into mouse zygotes with 100% efficiency. CRISPR-EZ enables highly efficient and high-throughput genome editing in vivo, with a significant improvement in embryo viability compared with microinjection-based technology